ABSTRACT
Objective:To investigate the expression of IL-37 and IL-18 in the lung tissue of rats with bleomycin induced pulmonary fibrosis,and to explore the expression and significance of PF in the lung. Methods:45 Sprague Wistar rats were randomly divided into normal control group(N Group),bleomycin group(B group),dexamethasone group(D group),15 rats in each group. B group and D group rats were given intratracheal injection of bleomycin 4 mg/kg,pulmonary fibrosis model was made,N group was given the same volume of normal saline as control group D,rats in pulmonary fibrosis model based on second days of daily intraperitoneal injection of dexamethasone 3 mg/kg;N, B group rats were intraperitoneally injected with normal saline as control. The rats were sacrificed after modeling 7,14,28 days,HE staining was used to evaluate the lung tissue pathological changes;hydroxyproline in lung tissue was measured by alkali hydrolysis and enzyme linked immunosorbent assay(HYP),the content of IL-37,the determination of ex-pression of IL-18mRNA in lung tissue by RT-PCR. Results:①HE staining showed that B, D group of rat lung tissue pathological changes by alveolar inflammation gradually developed into liver fibrosis;B,D group HYP content increased gradually with the elapse of time,for 28 days(P<0. 05).②B,D group IL-37,the expression of IL-18mRNA on the seventh day increased to the highest point. After gradually reduced,to 28 days were higher than N group,the difference was statistically significant(P<0. 05).③The expression of IL-37 and IL-18mRNA in group D was lower than that in group B at the same time(P<0. 05). Conclusion:IL-37,IL-18 play an important role in the early stage of pulmonary fibrosis in rats,and may be involved in the occurrence and development of pulmonary fibrosis.
ABSTRACT
Objective:To investigate the expression of IL-37 and IL-18 in the lung tissue of rats with bleomycin induced pulmonary fibrosis,and to explore the expression and significance of PF in the lung. Methods:45 Sprague Wistar rats were randomly divided into normal control group(N Group),bleomycin group(B group),dexamethasone group(D group),15 rats in each group. B group and D group rats were given intratracheal injection of bleomycin 4 mg/kg,pulmonary fibrosis model was made,N group was given the same volume of normal saline as control group D,rats in pulmonary fibrosis model based on second days of daily intraperitoneal injection of dexamethasone 3 mg/kg;N, B group rats were intraperitoneally injected with normal saline as control. The rats were sacrificed after modeling 7,14,28 days,HE staining was used to evaluate the lung tissue pathological changes;hydroxyproline in lung tissue was measured by alkali hydrolysis and enzyme linked immunosorbent assay(HYP),the content of IL-37,the determination of ex-pression of IL-18mRNA in lung tissue by RT-PCR. Results:①HE staining showed that B, D group of rat lung tissue pathological changes by alveolar inflammation gradually developed into liver fibrosis;B,D group HYP content increased gradually with the elapse of time,for 28 days(P<0. 05).②B,D group IL-37,the expression of IL-18mRNA on the seventh day increased to the highest point. After gradually reduced,to 28 days were higher than N group,the difference was statistically significant(P<0. 05).③The expression of IL-37 and IL-18mRNA in group D was lower than that in group B at the same time(P<0. 05). Conclusion:IL-37,IL-18 play an important role in the early stage of pulmonary fibrosis in rats,and may be involved in the occurrence and development of pulmonary fibrosis.